Acid-Base Properties of Amino Acids
Denaturing and Precipitating Proteins
Enzymatic Activity of Glucose Oxidase
HIV-1 Protease: An Enzyme at Work
Studying Enzyme Kinetics Using Catalase
Binding of Coomassie Brilliant Blue to Egg Albumin
Breaking Down Sucrose Using Invertase
Color Reactions of Amino Acids
Effect of pH on Protein Solubility
Enzyme Catalyzed Decomposition of Hydrogen Peroxide
Enzyme Kinetics: Experiments With Catalase
Halting the Briggs-Rauscher Reaction
Hydrolysis of Wool in Strong Base
Dyeing of Wool With Martius Yellow
Precipitating Genomic DNA
Description: DNA is precipitated by the addition of sodium acetate and ethanol. The precipitated DNA can be wound on a glass rod and removed from solution. This demonstration can be expanded to show the precipitation of DNA that was extracted from banana or onion.
Source: Kristin Johnson
Year: 1998 Vol: N/A Page: N/A
Keywords: Deoxyribnucleic Acid, Precipitation, Sodium Acetate, Ethanol
Rating: 
Hazard: Some
- Flammable liquids
- Eye irritation hazard
Effectiveness: Average
- Results are observable with guidance
- Somewhat connected to course material
- Mild effects are seen by audience
- Moderate failure rate
- Time to results is low
Difficulty: Low
- Simple addition of reagents
- Simple manipulations for most to perform
- Experiments using a display or video feed
Safety Precautions:
- Eye protection required
- Gloves required
- No open flames during presentation
- Perform in a well-ventilated area
- ABC fire extinguisher on hand
- Absorbent material on hand
Class: Biochemistry, Solubility
Division: General, Biological Chemistry
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