Acid-Base Properties of Amino Acids
Denaturing and Precipitating Proteins
Enzymatic Activity of Glucose Oxidase
HIV-1 Protease: An Enzyme at Work
Studying Enzyme Kinetics Using Catalase
Binding of Coomassie Brilliant Blue to Egg Albumin
Breaking Down Sucrose Using Invertase
Color Reactions of Amino Acids
Effect of pH on Protein Solubility
Enzyme Catalyzed Decomposition of Hydrogen Peroxide
Enzyme Kinetics: Experiments With Catalase
Halting the Briggs-Rauscher Reaction
Hydrolysis of Wool in Strong Base
Dyeing of Wool With Martius Yellow
Gel Filtration Chromatography
Description: Gel filtration chromatography is used to separate large molecules on the basis of size. Two columns are run simultaneously. The first column contains Sephadex G-75, which separates blue dextran and hemoglobin. The second column contains Sephadex G-10, which separates hemoglobin and riboflavin. Because there is a difference in two packing materials, the hemoglobin molecule runs very differently in two columns.
Source: Kristen Johnson
Year: 1998 Vol: N/A Page: N/A
Keywords: Gel filtration Chromatography, Hemoglobin, Riboflavin
Rating: 
Hazard: Low
- No use of toxic chemicals
- Standard lab conditions
Effectiveness: Good
- Results are clearly observable without guidance
- Good connection from demo to course material
- Clear contrast between systems behavior
- Moderate failure rate
- Time to results is medium
Difficulty: Medium
- Adding reagents in concert
- Procedures with some intermediate steps
- Sequential addition required
- Use of scientific equipment
- Prior practice recommended
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent materials on hand
Class: Biochemistry, Chromatography, Macromolecules and Solid State
Division: General, Analytical Chemistry, Biological Chemistry
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