Biochemistry- Studying Enzyme Kinetics Using Catalase Extract

Studying Enzyme Kinetics Using Catalase Extract

Description: Catalase activities are measured by timing the rise of enzyme-soaked piece of filter paper to the top of beakers containing different concentration of hydrogen peroxide. The data is plotted on a Lineweaver-Burk double reciprocal plot.

Source: Kristen Johnson

Year: 2000   Vol: N/A   Page: N/A

Keywords: Catalase, Hydrogen peroxide, LineWeaver-Burk, Concentration, Kinetics

Rating:

Hazard: Some

• Use of mildly reactive chemicals
• Oxidizer
• Mild toxicity hazard
• Aquatic toxicity hazard
• Skin corrosion hazard
• Eye damage hazard

Effectiveness: Average

• Results are observable with guidance
• Mild effects are seen by audience
• Good connection to course material
• Time to results is high

Difficulty: Medium

• Some concerted or timed manipulations
• Use of mildly reactive substances
• Use of scientific equipment
• Experiments in which a display is used
• Procedures with multiple steps to result

Safety Precautions:

• Eye protection required
• Gloves required
• Absorbent material on hand
• Sodium bicarbonate on hand
• Prevent release of reagents to the environment

Class: Biochemistry, Kinetics

Division: General, Physical Chemistry

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