Acid-Base Properties of Amino Acids
Denaturing and Precipitating Proteins
Enzymatic Activity of Glucose Oxidase
HIV-1 Protease: An Enzyme at Work
Studying Enzyme Kinetics Using Catalase
Binding of Coomassie Brilliant Blue to Egg Albumin
Breaking Down Sucrose Using Invertase
Color Reactions of Amino Acids
Effect of pH on Protein Solubility
Enzyme Catalyzed Decomposition of Hydrogen Peroxide
Enzyme Kinetics: Experiments with Catalase Extract
Halting the Briggs-Rauscher Reaction
Hydrolysis of Wool in Strong Base
Dyeing of Wool With Martius Yellow
Denaturing and Precipitating Proteins
Description: Acid, heat, and heavy metal ions such as lead all change the nature of egg white, illustrating protein denaturation. Proteins can be precipitated with ammonium sulfate and still remain active.
Source: UW Card Catalog
Year: N/A Vol: N/A Page: N/A
Keywords: Denaturation, Protein, Egg white, Precipitation
Rating: 
Hazard: Medium
- Acute toxicity hazard
- Skin corrosion hazard
- Inhalation hazard
- Aquatic toxicity hazard
- Flammability hazard
- Electric shock hazard
- Corrosive to metals
- Oxidizing solids
- Carcinogenicity
- Reproductive toxicity
- Specific organ toxicity – kidneys, central nervous system, blood, immune system
Effectiveness: Good
- Results are clearly observable without guidance
- Good connection to course material
- Low failure rate
Difficulty: Medium
- Procedures with some intermediate steps to results
- Demos at non-standard conditions
- Some sequential manipulations
Safety Precautions:
- Eye protection required
- Gloves required.
- Thermal gloves recommended
- UL approved three-prong plug and outlet
- ABC fire extinguisher on hand
- Absorbent materials on hand
- Perform in a well-ventilated area
- Prevent release of reagents to the environment
Class: Biochemistry, Denaturation
Division: General, Biological Chemistry
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