Chromatography of Spinach Extract
Extraction of Metals With Dithizone
Partitioning of Iodine in Solution
Computer Simulation of Mass Spectroscopy
Magic Sand Models the Hydrophobic Effect
Reverse Phase Separation of Dyes
Water Softening- Hard and Soft Water With Soap
Gel Filtration Chromatography
Two tubes filled with a pale solid over two flasks, one with a blue band and one with a brown band
Description: Gel filtration chromatography is used to separate large molecules on the basis of size. Two columns are run simultaneously. The first column contains Sephadex G-75, which separates blue dextran and hemoglobin. The second column contains Sephadex G-10, which separates hemoglobin and riboflavin. Because there is a difference in two packing materials, the hemoglobin molecule runs very differently in two columns.
Source: Kristen Johnson
Year: 1998 Vol: N/A Page: N/A
Keywords: Gel filtration Chromatography, Hemoglobin, Riboflavin
Rating: 
Hazard: Low
- No use of toxic chemicals
- Standard lab conditions
Effectiveness: Good
- Results are clearly observable without guidance
- Good connection from demo to course material
- Clear contrast between systems behavior
- Moderate failure rate
- Time to results is medium
Difficulty: Medium
- Adding reagents in concert
- Procedures with some intermediate steps
- Sequential addition required
- Use of scientific equipment
- Prior practice recommended
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent materials on hand
Class: Biochemistry, Chromatography, Macromolecules and Solid State
Division: General, Analytical Chemistry
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