Gel Filtration Chromatography
Description: Gel filtration chromatography is used to separate large molecules on the basis of size. Two columns are run simultaneously. The first column contains Sephadex G-75, which separates blue dextran and hemoglobin. The second column contains Sephadex G-10, which separates hemoglobin and riboflavin. Because there is a difference in two packing materials, the hemoglobin molecule runs very differently in two columns.
Source: Kristen Johnson
Year: 1998 Vol: N/A Page: N/A
Keywords: Gel filtration Chromatography, Hemoglobin, Riboflavin
Rating: 
Hazard: Low
- No use of toxic chemicals
- Standard lab conditions
Effectiveness: Good
- Results are clearly observable without guidance
- Good connection from demo to course material
- Clear contrast between systems behavior
- Moderate failure rate
- Time to results is medium
Difficulty: Medium
- Adding reagents in concert
- Procedures with some intermediate steps
- Sequential addition required
- Use of scientific equipment
- Prior practice recommended
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent materials on hand
Class: Biochemistry, Chromatography, Macromolecules and Solid State
Division: General, Analytical Chemistry
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