Spectroscopy- Optical Activity of Racemic Mixtures With Limonene

Optical Activity of Racemic Mixtures With Limonene

Description: A sample of S-(+)- limonene is placed in a large cuvette glass made for this experiment and placed between two polarizers on an overhead projector. the optical activity is noted by the prismatic effect that occurs while changing the relative position of the two polarizers filter lines.

Source: Shakhashiri, B.Z. Chemical Demonstrations: A Handbook for Teachers of Chemistry, Bowman, Matt  Dr.

personal consult (2018)

Year: 1989 Vol. 3    Page386 – 398

Keywords: Limonene, Polarized light.  Optical activity,  Racemic mixture,  Isomer, Enantiomer

Rating:

Hazard: Some

  • Flammable liquids
  • Skin sensitization hazard
  • Aspiration hazard
  • Short-term (acute) aquatic hazard
  • Long-term (chronic) aquatic hazard
  • Skin irritation hazard
  • Electric shock hazard
  • Breakage hazard

Effectiveness: Good

  • Primary effects are observed
  • Results are observable with guidance
  • Good connection from demo to course material
  • Time to results is medium
  • High reliability

Difficulty: Medium

  • Some careful manipulation required
  • Some intermediate steps to results
  • Use of toxic reagents
  • Use of electrical equipment

Safety Precautions:

  • Eye protection required
  • Gloves required
  • PPE required
  • Avoid inhalation of mists, droplets, or vapors
  • Use of UL approved three-prong plug and outlet
  • ABC fire extinguisher on hand
  • Prevent release of reagents to the environment
  • Absorbent materials on hand
  • Perform in a well-ventilated area
  • Prior training recommended
  • Observation distance one meter minimum

Class: Spectroscopy

Division: General, Organic Chemistry