Studying Enzyme Kinetics Using Catalase Extract
Description: Catalase activities are measured by timing the rise of enzyme-soaked piece of filter paper to the top of beakers containing different concentration of hydrogen peroxide. The data is plotted on a Lineweaver-Burk double reciprocal plot.
Source: Kristen Johnson
Year: 2000 Vol: N/A Page: N/A
Keywords: Catalase, Hydrogen Peroxide, Lineweaver-Burk, Concentration, Kinetics
Rating: 
Hazard: Some
- Use of mildly reactive chemicals
- Oxidizer
- Mild toxicity hazard
- Aquatic toxicity hazard
- Skin corrosion hazard
- Eye damage hazard
Effectiveness: Average
- Results are observable with guidance
- Mild effects are seen by audience
- Good connection to course material
- Time to results is high
Difficulty: Medium
- Some concerted or timed manipulations
- Use of mildly reactive substances
- Use of scientific equipment
- Experiments in which a display is used
- Procedures with multiple steps to results
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent material on hand
- Sodium bicarbonate on hand
- Prevent release of reagents to the environment
Class: Biochemistry, Kinetics
Division: General, Physical Chemistry
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