Chromatography and Separation- Double Beam Spectrophotometer

Double Beam Spectrophotometer

Description: A large scale setup of double beam spectrophotometer complete with splitter. The laser beam is passed through either a tank of colored water and onto a screen, or it is reflected around the tank by the splitter and onto the screen. The difference in the amount of light reaching the screen is the absorbance of the sample. Source: Kristen Johnson Year: 1999   Vol: N/A   Page: N/A Keywords: Spectrophotometer, Splitter, Light, Absorbance Rating: Hazard: Some
  • Coherent light hazard – eyes
Effectiveness: Average
  • Results are observable with guidance
  • Mild effects are seen by audience
  • Somewhat connected conceptually to course material
  • Moderate failure rate
Difficulty: Medium
  • Procedures with some intermediate steps to results
  • Prior training recommended
  • Complex lab equipment manipulations
Safety Precautions:
  • Eye protection required
  • Gloves required
  • Avoid exposure to coherent light
  • Absorbent material on hand
Class: Spectroscopy Division: Analytical Chemistry  

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