Chromatography of Spinach Extract
Description: Spinach extract is separated by thin layer chromatography into chlorophyll and B-carotene.
Source: Journal of Chemical Education
Year: 1985 Vol: 62 Page: 530
Keywords: Spinach, Chlorophyll, Chromatography
Rating: 
Hazard: High
- Flammability hazard
- Acute toxicity hazard
- Aspiration hazard
- Germ cell mutagenicity hazard
- Skin irritation
- Carcinogenicity
- Organ toxicity – central nervous system, kidneys
Effectiveness: Average
- Hard to see or perceive result
- Time to results is high
- Mild effects are seen by audience
- Results are observable with guidance
- Moderate failure rate
Difficulty: Medium
- Some timed manipulations
- Procedures with some intermediate steps to result
- Handling of organic chemicals
- Experiments in which display is required
Safety Precautions:
- Eye protection required
- Gloves required
- Lab coat recommended
- Perform preparation in hood
- Perform presentation in a well-ventilated area
- ABC fire extinguisher on hand
- Avoid exposure to open flame
- Absorbent materials on hand
- Avoid exposure to droplets, mists, or vapors
Class: Chromatography
Division: General, Analytical Chemistry, Organic Chemistry
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