Enzymatic Activity of Glucose Oxidase
Description: Some basic principles of enzymology are studied using the enzyme glucose oxidase. The reactions are varied by temperature, enzyme concentration, substrate concentration and substrate specificity, and type of substrate used. The reactions are monitored by the appearance of a chromogenic substrate coupled by the glucose oxidase reaction.
Source: Kristen Johnson
Year: 1999 Vol: N/A Page: N/A
Keywords: Enzyme glucose oxidase, Chromogenic, Substrate concentration, Specificity
Rating: 
Hazard: Some
- Electric shock hazard
- Acute toxicity hazard – oral
- Corrosive to metals
- Skin corrosion hazard
- Serious eye damage
Effectiveness: Average
- Results are observable with guidance
- Mild effects are seen by the audience
- Noticeable contrast between systems behavior
- Time to results is high
- Secondary effects are observed
Difficulty: High
- Multi-step procedures
- Complex lab equipment manipulations involving multiple steps
- Demos require training or extended practice
- Exacting preparation and use of reagents required
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent materials on hand
- Use of UL approved three-prong plug and outlet
- Avoid exposure to droplets and mists
Class: Biochemistry, Buffers, Indicators, Enzymes
Division: General, Biological Chemistry
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