Reverse Phase Separation of Dyes
Description: The “Sep Pak” C18 column and disposable syringe set is used to demonstrate liquid chromatography. Green food coloring is separated into its component parts by changing the methanol concentration in the liquid phase.
Source: Journal of Chemical Education- Vol. 62
Year: 1985 Vol: 62 Page: 798
Keywords: Sep Pak, Green food coloring, Methanol, Separation, Chromatography
Rating: 
Hazard: Some
- Acute toxicity hazard
- Flammability hazard
- Serious eye damage
Effectiveness: Average
- Mild effects are seen by audience
- Somewhat connected conceptually to course material
- Moderate failure rate
- Time to results is medium
- Effects are observable with guidance
Difficulty: Medium
- Some concerted or timed manipulations
- Procedures with some intermediate steps
- Use of syringe
- Prior training recommended
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent material on hand
- ABC fire extinguisher on hand
- Avoid exposure to mists, droplets and vapors
Class: Chromatography
Division: Analytical Chemistry
Home| Analytical Chemistry Demos