Precipitating Genomic DNA
Description: DNA is precipitated by the addition of sodium acetate and ethanol. The precipitated DNA can be wound on a glass rod and removed from solution. This demonstration can be expanded to show the precipitation of DNA that was extracted from banana or onion.
Source: Kristin Johnson
Year: 1998 Vol: N/A Page: N/A
Keywords: Deoxyribnucleic acid, Precipitation, Sodium Acetate, Ethanol
Rating: 
Hazard: Some
- Flammable Liquids
- Eye irritation hazard
Effectiveness: Average
- Results are observable with guidance
- Somewhat connected to course material
- Mild effects are seen by audience
- Moderate failure rate
- Time to results is low
Difficulty: Low
- Simple addition of reagents
- Simple manipulations for most to perform
- Experiments using a display or video feed
Safety Precautions:
- Eye protection required
- Gloves required
- No open flames during presentation
- Perform in a well-ventilated area
- ABC fire extinguisher on hand
- Absorbent material on hand
Class: Biochemistry, Solubility
Division: General, Biological Chemistry
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