Binding of Coomassie Brilliant Blue to Egg Albumin
Description: Acidified Coomassie Brilliant Blue dye changes from reddish-brown to blue when it binds to protein molecules. The degree of color changes is proportional to the amount of protein added. By adding varying amounts of protein to an acidified dye solution, these color changes can be observed.
Source: Kristin Johnson
Year: 1999 Vol: N/A Page: N/A
Keywords: Coomassie Brilliant Blue, Proteins, Egg albumin, Stoichiometry
Rating: 
Hazard: Some
- Acute toxicity hazard
- Serious eye damage
- Skin corrosion
- Acute aquatic toxicity
Effectiveness: Average
- Somewhat connected conceptually to course material
- Mild effects are seen by audience
- Moderate failure rate
- Time to results is medium
Difficulty: Medium
- Volume-dependent addition of reagents
- Procedures with some intermediate steps
- Prior training recommended
- Some careful manipulations required
Safety Precautions:
- Eye protection required
- Gloves required
- Absorbent materials on hand
- Sodium bicarbonate on hand
- Prevent release of reagents to the environment
Class: Biochemistry
Division: General, Biological Chemistry
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