Biochemistry- Studying Enzyme Kinetics Using Catalase Extract

 Studying Enzyme Kinetics Using Catalase Extract

Description: Catalase activities are measured by timing the rise of enzyme-soaked piece of filter paper to the top of beakers containing different concentration of hydrogen peroxide. The data is plotted on a Lineweaver-Burk double reciprocal plot. Source: Kristen Johnson Year: 2000   Vol: N/A   Page: N/A Keywords: Catalase, Hydrogen Peroxide, LineWeaver-Burk, Concentration, Kinetics Rating: Hazard: Some
  • Use of mildly reactive chemicals
  • Oxidizer
  • Mild toxicity hazard
  • Aquatic toxicity hazard
  • Skin corrosion hazard
  • Eye damage hazard
Effectiveness: Average
  • Results are observable with guidance
  • Mild effects are seen by audience
  • Good connection to course material
  • Time to results is high
Difficulty: Medium
  • Some concerted or timed manipulations
  • Use of mildly reactive substances
  • Use of scientific equipment
  • Experiments in which a display is used
  • Procedures with multiple steps to result
Safety Precautions:
  • Eye protection required
  • Gloves required
  • Absorbent material on hand
  • Sodium bicarbonate on hand
  • Prevent release of reagents to the environment
 
Class: Biochemistry, Kinetics Division: General, Physical Chemistry, Biological Chemistry

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